Xenbase Image ID: 49409

Fig. 7. BrdU incorporation demonstrates reduced cell proliferation following FoxO overexpression and depletion. (A) Animal view of control embryo, stage 9.5. GOF embryos were bilaterally injected at 2-cell stage with 500 pg/blastomere FoxO1 or FoxO3, 250 pg/blastomere FoxO3-TM, 400 pg/blastomere FoxO4 and 200 pg/blastomere FoxO6 RNA. (B) Bilaterally CoMO and FoxO3MO injected embryos at stage 11.5 (animal view) and stage 15 (dorsal view). (C) CoMO, FoxO1MO and FoxO6MO injected embryo at stage 28 (lateral view). (D) Unilaterally FoxO4MO injected embryo at stage 27. (E) Magnification of the head of an unilaterally FoxO4MO injected embryo at stage 30 (lateral view). Morpholinos were uni- or bilaterally injected at indicated amounts into 2-cell stage embryos. Note that the blue spots represent BrdU positive cells, while the brown color is due to natural pigmentation of embryos. Asterisks indicate the morpholino injected side. ba, branchial arches; e, eye; n, neural fold. Image published in: Schuff M et al. (2010) Copyright © 2010. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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