Xenbase Image ID: 49453

FIG. 4. Histological analysis of enlarged and ectopic pronephroi. Dorsal is up in all samples. (A) Control. Transverse section through a normal pronephros, pronephric tubules stained with 3G8 in dark blue and pronephric duct stained with 4A6 in light blue. (B) Enlarged pronephros. The pronephros on the injected side of the same embryo as shown in A. This sample developed from an embryo injected with XPax-8, Xlim-1, and b-galactosidase mRNA. The lineage tracer was developed with a red substrate and can be observed in the epithelia of the enlarged pronephric tubules. Once again, tubules are stained dark blue, and duct stained light blue. The red tracer stain can be clearly visualized in tubules due to the restriction of the 3G8 epitope to the apical surface of tubule epithelia. (C) Ectopic pronephric tubule. The ectopic tubules (arrowhead) are stained with 3G8 and a dark blue substrate and contain the b-galactosidase lineage tracer (red). Somites were stained with 12/101 and a light blue substrate. Note the reduction in size of the somites on the left side below the ectopic tubules. (D) Ectopic pronephric tubule. A sample processed in the same manner as that shown in C. Both normal (b-galactosidase negative) and ectopic (b-galactosidase positive, arrowhead) pronephric tubules are visible on the left side. Once again, the somites are smaller on the side with the ectopic pronephric tubules. S, somite; N, notochord. Image published in: Carroll TJ and Vize PD (1999) Copyright © 1999. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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