Xenbase Image ID: 49518

Figure 7. Rescue of BMP2/4/7/ADMP-Depleted Embryos by BMP4 Protein and Demonstration that Ventral and Dorsal Centers Serve as BMP Sources In Vivo. (A) Bmp2/4/7/Admp MOs neuralize the entire ectoderm (n = 25 or more per experimental set). (B) Epidermal fate and DV pattern are rescued in BMP2/4/7/ADMP-depleted embryos by injection of recombinant BMP4 protein (1.5 ng in 40 nl). (C) Experimental design of blastomere lineage-tracing experiments. (D) B4 blastomere descendants are distributed along the AP axis of BMP2/4/7/ADMP-depleted embryos (n = 17). (E) DV pattern in BMP2/4/7/ADMP-depleted embryos is rescued by BMP4 protein injection (n = 56) according to the initial DV polarity of the fertilized egg, as demonstrated by nuclear LacZ staining in the B4 lineage. (F) Experimental procedure in which wild-type grafts (marked by nuclear lacZ) were transplanted into ADMP/BMP2/4/7-depleted hosts at early gastrula. (G) Ventral-center grafts (inset) rescue neural plate and epidermal differentiation over many cell diameters (n = 19; for control nongrafted hosts, see [A] and Figure 6F). This experiment demonstrates that the ventral center produces organizing signals. (J) Dorsal-center grafts elongate and rescue neural and epidermal patterning over the entire embryo (n = 21). The dorsal graft is the sole source of BMP in these quadruple BMP2/4/7/ADMP-depleted hosts. Note that epidermis is induced only at a distance. Image published in: Reversade B and De Robertis EM (2005) Copyright © 2005. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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