XB-IMG-49519
Xenbase Image ID: 49519
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Figure 1. GSK3b Is Translocated to Acidic Vesicles upon Wnt Signaling
(A) In coculture experiments, xWnt8-Venus (green) secreted by 293T cells caused the relocalization of GSK3b-RFP (red) in 3T3 cells that endocytosed Wnt.
Arrows in the enlargement in (F) indicate xWnt8-Venus endosomes that sequester GSK3-RFP. In controls lacking xWnt8-Venus, GSK3-RFP localizationwas cytoplasmic
(D and E); asterisks indicate untransfected control 293 cell nuclei.
(G0 ) GSK3b antigen relocalized to LysoTracker-positive endosomes upon activation of Wnt pathway by CA-LRP6 in 3T3 cells; note that endogenous GSK3b
was depleted from cytoplasm.
(M0 ) Rab7-GFP (green) and GSK3b-RFP (red) colocalized in CA-LRP6 signalosomes (in 62% 7% of GSK3-positive puncta, n = 80 HeLa cells); note GSK3
sequestration from cytosol.
(S) Endogenous GSK3b puncta (white) were larger and more numerous after 4 hr of Wnt3a addition to untransfected 3T3 cells permeabilized with Digitonin.
Data are represented as mean SEM. Also see Figure S1 and Figure S2. Image published in: Taelman VF et al. (2010) Copyright © 2010. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |