XB-IMG-49521
Xenbase Image ID: 49521
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Figure 3. Components of the ESCRT Machinery Are Required for Wnt Signaling
(A) HRS and Vps4 are proteins required for intralumenal vesicle formation in MVBs. GSK3 is indicated in red.
(B) HRS siRNA inhibits Wnt3a-induced accumulation of b-Catenin.
(C) TCF-Luciferase reporter gene assays showing that Wnt signaling requires HRS (brackets).
(D0 ) HRS is required for sequestration of GSK3-RFP in CA-LRP6 signalosomes (78% 8%, n = 150).
(F) Wnt signaling induced by CA-LRP6 mRNA in Xenopus animal caps was blocked by HRS morpholino (brackets). (G) Induction of secondary axis in Xenopus
embryos by CA-LRP6 mRNA (80 pg) requires HRS (neural tissue visualized with Sox2 probe).
(J and K) Expression of Vps4-EQ, but not Vps-WT, inhibited signaling by Wnt3a or CA-LRP6 (brackets) in 293T cells.
(L0 ) CA-LRP6 signalosomes sequestered endogenous GSK3b and partly colocalized with Vps4-WT-GFP, a multivesicular endosome marker (43% 5% of
vesicles, arrows, in n = 80 cells).
(O0 ) Overexpression of Vps4-EQ-GFP inhibits sequestration of endogenous GSK3b in CA-LRP6 signalosomes. (R) Head formation in axes induced by
CA-LRP6 was inhibited by Vps4-EQ, but not by Vps4-WT mRNA.
Data are represented as mean SEM. Also see Figure S3 and Figure S4. Image published in: Taelman VF et al. (2010) Copyright © 2010. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |