XB-IMG-49527
Xenbase Image ID: 49527
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Figure 5. FRL1 Is Required for Dorsal Axis Formation
(A) Embryos derived from FRL1-depleted and control oocytes frozen as matured oocytes and as embryos at the stages shown and assayed
by real-time RT-PCR for the relative expression of FRL1 mRNA. C = control; L = 1.5 ng mp9; H = 2 ng mp9.
(B) Early tailbud embryos derived from control and FRL1-depleted oocytes (1, 1.5, 2 ng oligo injected).
(C) Sibling embryos to those shown in (B) were frozen at the early gastrula stage and assayed by real-time RT-PCR for the relative expression
of Wnt target genes.
(D) The ventralization of FRL1-depleted embryos is partially rescued by the injection of 50 pg FRL1 mRNA.
(E and F) The relative expression level of Wnt and VegT target genes in control, FRL1-depleted, and FRL1-depleted + 50 pg FRL1 mRNA
injected sibling embryos (to those shown in [D]) at the stages shown. Image published in: Tao Q et al. (2005) Copyright © 2005. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |