Xenbase Image ID: 49544

Figure 1. Morpholinos Targeting Two Distinct chordin mRNAs Reduce Chordin Expression and Promote Ventralization (A) Sequence of the morpholinos targeting the two Chordin mRNAs. (B) Western blot analysis of secreted Chordin protein. Two-cell embryos were injected four times with a total of 8 ng of Chd-MO-1 (lane 2) or Chd-MO-2 (lane 3) or with a combination of the two (4 ng each; lane 4). Dorsal lips were isolated at gastrula, and explants were cultured in Ca2 -Mg2 -free medium (CMFM) for 12 hr. The supernatant was then analyzed in western blots with an immunopurified anti-Chordin antibody; Coomassie blue staining of the cell pellets served as loading control. (C) Embryos microinjected four times at the two-cell stage with a total of 8 ng of each Chordin morpholino or a mixture of the two. The ventralized phenotype observed by coinjection of the two morpholinos generated a highly penetrant ventralized phenotype ( 75%) in many independent experiments. (F) The effect of Chd-MO(1 2) was rescued by microinjection of 10 pg chd mRNA lacking the 5 UTR. Image published in: Oelgeschläger M et al. (2003) Copyright © 2003. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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