Xenbase Image ID: 49547

Figure 4. Chordin Is Required for the Dorsalization of Xenopus Embryos by LiCl Treatment(A) Stage 45 untreated tadpole.(B) Sibling embryo treated with 120 mM LiCl at the 32-cell stage for 30 min.(C) Stage 45 embryo microinjected into the animal pole with 200 pg chordin mRNA at the four-cell stage.(D) Chordin production is inhibited by Chd-MO(1+2), even after LiCl treatment (compare lanes 2 and 3). Whole embryos were dechorionated and dissociated at stage 9 and cultured for 14 hr at 19°C in CMFM to allow detection of Chordin protein in the supernatant.(E and F) LiCl-treated embryos display a radial cement gland at tadpole stages and do not develop trunk-tail structures (dorso anterior index [DAI] = 8, n = 73)(G) Embryos at the two-cell stage with 8 ng Chd-MO(1+2) before LiCl treatment are resistant to LiCl (G; DAI = 6.4, n = 38). Image published in: Oelgeschläger M et al. (2003) Copyright © 2003. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

Return to previous page