Xenbase Image ID: 49550

Figure 7. The Mesodermal Specification of Dorsal Lip Explants Is Not Changed by Chd-MO(1 2) Microinjection (A) Explanted dorsal lips (n 25) developed into anterior neural tissue (eye, ne), notochord (no), endoderm (en), and cement gland (cg) at stage 38. (B) Chd-MO(1 2)-injected dorsal lip explants (n 27) developed into notochord (no), muscle (mu), posterior neural tissue (ne), otic vesicle (ov), and epidermis (epi). (C and D) Uninjected and Chd-MO(1 2)-injected dorsal lip explants were positive for the notochord-specific antibody MZ15 (n 5). (E and F) Uninjected and Chd-MO(1 2)-injected dorsal lip explants were stained by the muscle-specific antibody 12-101. (G) RT-PCR analysis of dorsal lip explants at stage 10.5 showing that organizer gene expression (chd, noggin, follistatin, and cerberus) was not affected by Chd-MO(1 2) at the time of the transplantations. (H) RT-PCR analysis of Chd-MO(1 2)-injected dorsal lip explants at stage 26. In Chd-MO(1 2)-microinjected embryos the anterior neural markers Six-3 and Rx2a were inhibited, the pan-neural marker NCAM and the cement gland marker XAG were reduced, and the notochord marker Xnot and the somite marker gene actin were not affected. Image published in: Oelgeschläger M et al. (2003) Copyright © 2003. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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