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Fig. 1. Protein sequence, chromosomal synteny and expression profile of Xenopus PouV genes. (A) Alignment of human (HsOCT4), mouse (MmOct4), Xenopus (Xlpou91, Xlpou25, Xlpou60), axolotl (AmOct4) and zebrafish (DrPou2) PouV proteins. POUs and POUh domains are overlined in red and blue, respectively. Pink indicates identical residues, light blue indicates similar residues, dark blue indicates conservation within a subset of residues. (B) Syntenic relationship between the Xenopus tropicalis sequence scaffold 302, zebrafish chromosome 16 and mouse chromosome 17. (C) Expression analysis of the XlpouV genes. Embryos on the right were bisected prior to in situ hybridization. The arrowheads indicate the dorsal lip.