Xenbase Image ID: 49564

Fig. 5. XlpouV depletion results in defective anterior and posterior neural patterning. (A) In situ hybridization for expression of Xlpou25 at neurula stages. Xlpou25 expression is visible in the developing neural tube at stages 13 and 14 and is then present in the midbrain hindbrain boundary (MHB) at stage 17. The arrowhead indicates the MHB. Xlpou91 showed an identical expression pattern (data not shown). (B) Embryos were injected with control MO or XlpouV MO as in Fig. 4C, and in situ hybridization carried out for Fgf8 expression at stage 16 and for En-2 and Krox20 expression at both stage 16 and stage 35. The insets show stage 16 embryos injected in one blastomere at the two-cell stage with MO and a lineage trace (orange). The arrowheads in the Fgf8 panel indicate the MHB. The arrowheads in the Krox20 panel indicate rhombomere 3 and 5 in the control MO-treated embryo and rhombomere 5 in the XlpouV MO-treated embryo. (C) In situ hybridization for expression of Xlpou25 at tailbud stages. The right panel is a transverse section through the posterior region of a stage 29 embryo. Xlpou91 showed an identical expression pattern (data not shown). (D) Depletion of XlpouV protein results in increased Xcad3 expression. Embryos were injected with control MO or XlpouV MO as in Fig. 4C, and in situ hybridization for Xcad3 expression performed on stage 28 embryos. (E) Whole-mount TUNEL staining. Embryos were injected with control MO or XlpouV MO as in Fig. 4C and TUNEL staining carried out at stage 11.5. Embryos are positioned with the blastopore to the bottom and both sides of the embryo are shown. The boxed areas are shown at higher magnification below the main images. Image published in: Morrison GM and Brickman JM (2006) Copyright © 2006. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue pou5f3.2.L Oct-25, oct25, pou25, xoct-25, xoct25 X. laevis Throughout NF stage 13 chordal neural plate pou5f3.2.L Oct-25, oct25, pou25, xoct-25, xoct25 X. laevis Throughout NF stage 14 chordal neural plate pou5f3.2.L Oct-25, oct25, pou25, xoct-25, xoct25 X. laevis Throughout NF stage 17 anterior dorsal lateral plate region presumptive midbrain-hindbrain boundary pou5f3.2.L Oct-25, oct25, pou25, xoct-25, xoct25 X. laevis Throughout NF stage 29 and 30 spinal cord fgf8.L AIGF, FGF-8, fgf-8b, fgf8a, fgf8b, MGC79739, Xfgf-8, Xfgf8 X. laevis Throughout NF stage 16 presumptive midbrain-hindbrain boundary posterior anterior neural ridge en2.L En-2, en2-a, en2-b, eng2, engrailed 2, engrailed homolog 2, engrailed-2, maben, xen2 X. laevis Throughout NF stage 35 and 36 midbrain-hindbrain boundary forebrain en2.L En-2, en2-a, en2-b, eng2, engrailed 2, engrailed homolog 2, engrailed-2, maben, xen2 X. laevis Throughout NF stage 16 presumptive midbrain-hindbrain boundary egr2.L EGR-2, Krox-20, krox20, XKr20, XKrox-20 X. laevis Throughout NF stage 16 rhombomere R3 rhombomere R5 egr2.L EGR-2, Krox-20, krox20, XKr20, XKrox-20 X. laevis Throughout NF stage 35 and 36 rhombomere R3 rhombomere R5 forebrain branchial arch cdx4.L cad3, MGC130769, MGC68909, Xcad-3, Xcad3, Xtcad3 X. laevis Throughout NF stage 28 posterior neural tube Image source: Published Larger Image Printer Friendly View

Return to previous page