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Fig. 6. Depletion of XlPouV proteins affects germ layer induction. (A) Two-cell stage embryos were injected in both blastomeres with 60 ng of control MO or 20 ng of each of the XlpouV MOs. In situs were performed on stage 10.25 embryos. Embryos are positioned with the vegetal hemisphere upwards and the dorsal lip towards the top apart from Bmp4 and Xom, which are positioned animal hemisphere upwards and insets with vegetal hemisphere upwards. (B) Real-time RT-PCR analysis of XlpouV protein-depleted embryos. Embryos were injected with control MO or XlpouV MO as in Fig. 6A and RNA extracted at stage 10.25. The relative change in gene expression was calculated by dividing the XlpouV MO values by the control MO values. Experiments were carried out in triplicate. (C) Embryos were injected with control MO or XlpouV MO as in Fig. 6A. Sox2 and Ngnr1 expression was analysed at stage 13 and Hex and MLC1-3 expression at stage 35 embryos. (D) Two-cell stage embryos were injected in both blastomeres with 250 pg of each RNA and in situs performed on stage 10.25 embryos. All embryos are positioned with the vegetal hemisphere facing upwards and the dorsal lip towards the top, apart from Bmp4 embryos that are positioned animal hemisphere upwards. Image published in: Morrison GM and Brickman JM (2006) Copyright © 2006. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue bmp4.L bmp-4, bmp2b, bmp2b1, bmp4-a, bmp4-b, DVR-4, ofc11, XBMP-4, xbmp4, zyme X. laevis Throughout NF stage 10.25 ventral marginal zone ventx2.2.L vent-2, vent2, Xom, xvent-2, xvent2 X. laevis Throughout NF stage 10.25 mesoderm tbxt.S bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. laevis Throughout NF stage 10.25 mesoderm fgf8.L AIGF, FGF-8, fgf-8b, fgf8a, fgf8b, MGC79739, Xfgf-8, Xfgf8 X. laevis Throughout NF stage 10.25 mesoderm mixer.L tMixer X. laevis Throughout NF stage 10.25 endomesoderm sox17a.L sox17 alpha, sox17-alpha, Sox17alpha, tSox17alpha, xsox17, Xsox17-alpha, xsox17a, Xsox17alpha, xSox17alpha1, xSox17alpha2 X. laevis Throughout NF stage 10.25 upper blastopore lip endoderm gsc.L goosecoid, gsc-a, gsc-b, Xgsc X. laevis Throughout NF stage 10.25 upper blastopore lip presumptive axial mesoderm neurog2.L atoh4, neurog2-a, neurog2-b, neurogenin 2, neurogenin-2, neurogenin-related-1, neurogenin2, ngn-2, ngn2, ngnr, ngnr 1a, Ngnr-1, Ngnr1, X-ngnr-1, xngn2, Xngnr-1, XNgnr1 X. laevis Throughout NF stage 13 anterior sox2.L anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Throughout NF stage 13 neural plate hhex.L hex, tHex, XHex X. laevis Throughout NF stage 35 and 36 foregut endoderm mlc1.L LOC108711162, lvm, mlc, mlc1-3 X. laevis Throughout NF stage 35 and 36 muscle somite Image source: Published Larger Image Printer Friendly View

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