Xenbase Image ID: 49574

Fig. 7. Lowering FGF8 levels causes a reduction in placode formation and neural differentiation. (A-E) X. laevis embryos displayed dorsoanteriorly. Embryos were cultured with XlMOF8 (10 ng) or MOSAF8a (60 ng) until the neural tube stage 20; lineage tracer (pink). (B,C) XlMOF8 caused sox2 mispatterning (40/42), so did MOSAF8a but to a lesser degree (20/22). (E) MOSAF8a resulted in a slight expansion of the rx1 domain toward the posterior (16/20). (F-M) The effect of FGF8 reduction on early neuronal differentiation in X. tropicalis. XtMOF8 (8 ng), MOSDF8 (17 ng) and MOSAF8a (16 ng) were injected into one cell at the two-cell stage; injected side is oriented downwards. (F,H,J,L) Embryos were cultured until neurula stages; injected embryos demonstrate an early strong reduction in neuronal differentiation [XtMOF8 (20/20), MOSDF8 (9/9), MOSAF8a (16/16)]. (G,I,K,M) Embryos were cultured until the early tadpole stage; injected embryos demonstrate posterior truncations and continued reduction in differentiated neurons Image published in: Fletcher RB et al. (2006) Copyright © 2006. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue sox2.S anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Throughout NF stage 20 neural tube rax.L rax-a, rax-b, rax1, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A X. laevis Throughout NF stage 20 optic vesicle tubb2b.L N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 20 neuron neuroectoderm Image source: Published Larger Image Printer Friendly View

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