Xenbase Image ID: 49608

Fig. S3. Wnt-4 overexpression promotes glomus formation and proximal tubulogenesis when co-injected with DeltaSTU.X. laevis embryos were injected into a V2 blastomere with a combination of wnt4 mRNA and deltaSTU mRNA, then left to develop to stage 34 where whole mount in situ hybridisation for nephrin and slc5a2 expression was performed. wnt4 overexpression with concomitant inhibition of Notch signalling caused ectopic nephrin (A) highlighting that Wnt-4 acts downstream of Notch signalling in the proximal pronephros. In addition, slc5a2 expression is not significantly reduced by co-injection of wnt4 deltaSTU and frequently the expression domain of slc5a2 is more distal (see dorsal view of pictured embryo). Asterisk denotes injected side. Image published in: Naylor RW and Jones EA (2009) Copyright © 2009. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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