Xenbase Image ID: 49609

Fig. S5. lfng misexpression inhibits pronephros formation, knockdown of rfng translation has no effect on pronephrogenesis.X. laevis embryos were injected at the eight-cell stage into a ventro-vegetal blastomere to target the presumptive pronephric region, with beta-galactosidase mRNA to act as a lineage tracer (blue staining on injected side). (A-D) Embryos were cultured to stage 41, when they were fixed, stained for the lineage label and correctly targeted embryos were whole-mount antibody stained for epitopes in the nephrostomes and proximal tubules, detected by 3G8 (purple stain, white arrow), and intermediate and distal tubules, detected by 4A6 (red stain, dashed white arrow). Injection of a control MO in addition to beta-galactosidase mRNA had no effect on pronephros development (A and E). Injection of lfng mRNA (B), lfng MO1 (C) and lfng MO2 (D) inhibited pronephrogenesis, as observed by reduced 3G8 and 4A6 staining. Additionally, we have observed the effects of lfng mis-expression on slc5a2, nephrin and Na+K+ATPase expression and found all domains of the pronephros did not form (data not shown). Injection of the rfng MO had no effect on pronephrogenesis, 3G8 and 4A6 staining was normal on the injected side. We also did not detect any effect of Rfng depletion on slc5a2, nephrin and Na+K+ATPase expression, showing all pronephric compartments formed (data not shown). Asterisk denotes injected side. Image published in: Naylor RW and Jones EA (2009) Copyright © 2009. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

Return to previous page