Xenbase Image ID: 49610

Fig. S6. Xbrachyury (t) expression is perturbed by lfng mis-expression. Since embryos injected with lfng mRNA or MOs had gross developmental phenotypes (such as a curled tail and shortness in length), we hypothesised that lfng mis-expression had earlier effects on development that affected pronephrogenesis indirectly. To determine this, X. laevis embryos were injected into both cells of a two-cell-stage embryo in the equatorial region with beta-galactosidase mRNA to act as a lineage tracer (red staining). Embryos were left to develop to stage 10-11, fixed, stained for the lineage label and whole-mount in situ hybridised for expression of a marker of the early mesoderm, Xbrachyury (Smith et al., 1991). (A) Injection of a control MO had no effect on Xbrachyury expression. (B-D) Overexpression of lfng (B) and depletion of lfng by injection of lfng MO1 (C) or lfng MO2 (D) statistically significantly reduced Xbrachury expression (P>0.05). These results confirm that Lfng mis-expression has early effects on mesodermal development that could be the cause of inhibited pronephrogenesis. Image published in: Naylor RW and Jones EA (2009) Copyright © 2009. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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