Xenbase Image ID: 49613

Fig. S1. FoxD3 expression in the Spemann Organizer. Whole-mount in-situ hybridization analysis of FoxD3 at gastrula stages. Vegetal views (dorsal up) of early gastrula (stage 10.25) (A) and mid-gastrula (stage 11.5) (B) embryos are shown. FoxD3 mRNA is expressed in the Spemann Organizer domain of the dorsal marginal zone that converges to the midline and extends along the AP axis during gastrulation. Whole-mount immunocytochemistry analysis of the FoxD3 protein in the gastrula using an affinity-purified anti-Xenopus FoxD3 polyclonal antibody. High-magnification views of the dorsal marginal zone (C) and the ventral marginal zone (D) of a cleared mid-gastrula embryo (stage 11) are shown (animal up). FoxD3 protein is detected in the nuclei of cells of the Spemann Organizer domain, but not in ventral cells. (E) Onset of endogenous FoxD3 expression at the gastrula stage as shown by RT-PCR analysis of Xenopus embryos at the indicated stages. Numerical stage, as defined by Nieuwkoop and Faber (Nieuwkoop and Faber, 1967), is indicated in parentheses. EF1α is a control for RNA recovery and loading. Intact embryos (Embryo) served as a positive control and an identical reaction without reverse transcriptase controlled for PCR contamination (Embryo-RT). Image published in: Steiner AB et al. (2006) Copyright © 2006. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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