XB-IMG-49614
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Fig. S4. FoxD3 rescue of axis formation in embryos injected with VP16-FoxD3 or FoxD3MO. The inhibition of axis formation by VP16-FoxD3 and FoxD3MO is predicted to result from a specific block of endogenous FoxD3 function. To determine the specificity of FoxD3 inhibition, FoxD3 was co-injected with VP16-FoxD3 or FoxD3MO in an attempt to rescue axis formation. At the four-cell stage, both dorsal blastomeres were injected with VP16-FoxD3 or FoxD3MO alone, or in combination with FoxD3 RNA, and axis formation was assessed at the tadpole stage. Whereas the majority of VP16-FoxD3-injected embryos had severe axial defects, only a minority displayed defects with FoxD3 co-injection (C,D). Similarly, the axial defects caused by FoxD3MO were rescued by FoxD3 RNA lacking the antisense target sequence (FoxD3-utr), but not by FoxD3 RNA containing the target sequence (FoxD3+utr) (E,F,H). As controls, injection of both dorsal blastomeres with FoxD3 RNA alone (B) or mismatch MO (G) did not perturb axis formation, by comparison with control (A). See Table 1 for quantification. Image published in: Steiner AB et al. (2006) Copyright © 2006. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |