XB-IMG-49616
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Fig. S2. Mesodermal and endodermal gene expression changes in embryos resulting from Geminin overexpression and morpholino knockdown. (A) 120 pg Gemininδcoil mRNA or 1 ng MO was co-injected with 20 pg β-galactosidase lineage tracer mRNA into one blastomere at the eight-cell stage and the embryos were raised to stage 11-11.5. After lineage labeling and in situ hybridization as before, embryos were selected for scoring only if the lineage-labeled region overlapped the marker gene. For each gene analyzed, numbers represent additive data from three to six experiments. (B) Dose dependence of Geminin knockdown effects. The three doses of Geminin MO indicated were injected into embryos and effects on Xpo, Sox17, Mix.2 and Xbra expression were quantitated as above; the fraction of embryos with the change in expression indicated is shown in each chart, with number of embryos scored in parentheses in the legend. (C) The effects of Geminin MO knockdown (1 ng injection) on Brachyury expression was scored for embryos at stage 10-11. Examples, with lineage-labeled territory indicated (pink stain, arrowhead) are shown at right. Geminin knockdown has no effect in many embryos at early gastrulation, and induces some ectopic Brachyury expression. By contrast, Geminin knockdown strongly reduces Brachyury expression in nearly all embryos by mid-late gastrulation. Image published in: Lim JW et al. (2011) Copyright © 2011. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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