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XB-IMG-49619

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Fig. S7. Oct25/60 and Sox2 suppression of mesoderm and endoderm is not blocked by Geminin knockdown. Embryos were injected with the reagents indicated, plus β-galactosidase mRNA, were lineage labeled (Red-gal, pink), and were in situ hybridized to detect Xpo, Brachyury or Sox17 (see Materials and methods). 120 pg of mRNAs (Oct25, Oct60, Sox2, Ezh2), 8 ng of Ezh2MO and Suz12MO, and 1 ng Geminin MO were used. Arrowheads mark injected regions. On the right, embryos were only scored if the lineage label was visible and overlapped expression of the marker; number of embryos analyzed is in parentheses.

Image published in: Lim JW et al. (2011)

Copyright © 2011. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

GeneSynonymsSpeciesStage(s)Tissue
post.LLOC108704914, XpoX. laevisThroughout NF stage 10.5ventro-lateral marginal zone
tbxt.Sbra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, XbrachyuryX. laevisThroughout NF stage 10.5mesoderm
marginal zone
sox17a.Lsox17 alpha, sox17-alpha, Sox17alpha, tSox17alpha, xsox17, Xsox17-alpha, xsox17a, Xsox17alpha, xSox17alpha1, xSox17alpha2X. laevisThroughout NF stage 12endoderm

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