Xenbase Image ID: 49626

Fig. S1. β-catenin activity represses expression of foregut genes hhex and foxa2. (A,B) At the 32-cell stage, embryos were injected either (A) in the D1 cell with stabilized pt-β-catenin RNA (250 pg) or (B) in the D4 cell with Gsk3β (500 pg) to repress Wnt signaling. At gastrula stage, either anterior endoderm (AE) or posterior endoderm (PE) was isolated and assayed after only 4 hours by RT-PCR for expression of hhex, foxa2 and the pan-endodermal marker sox17α. Bar charts showing normalized relative mRNA expression levels. (C-E) In situ hybridization of bisected stage-18 embryos with a foxa2 probe. Xenopus foxa2 is expressed throughout the endoderm but is enriched in the foregut. (D) Injection of stabilized pt-β-catenin RNA (250 pg) in D1 at the 32-cell stage results in partial repression of foxa2 in the foregut, whereas (E) injection of Gsk3β (500 pg) in D4 upregulates foxa2 expression in the posterior endoderm. Image published in: McLin VA et al. (2007) Copyright © 2007. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue foxa2.L HNF-3B, hnf3-beta, hnf3b, hnf3beta, LOC108717763 X. laevis Throughout NF stage 18 endoderm foregut Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + ctnnb1tm1 + endoderm explant + gastrula stage (RT-PCR) Fig.S.1.A Expression Phenotype decreased amount foxa2.L expression in anterior endoderm decreased amount hhex.L expression in anterior endoderm Xla Wt + gsk3b + endoderm explant + gastrula stage (RT-PCR) Fig.S.1.B Expression Phenotype increased amount foxa2.L expression in posterior endoderm Xla Wt + ctnnb1tm1 + NF18 (in situ hybridization) Fig.S.1.D Expression Phenotype decreased amount foxa2.L expression in foregut endoderm Xla Wt + gsk3b + NF18 (in situ hybridization) Fig.S.1.E Expression Phenotype increased amount foxa2.L expression in posterior endoderm Larger Image Printer Friendly View

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