Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-IMG-49627

Xenbase Image ID: 49627


Fig. S2. Vent2 is expressed in the posterior endoderm and can repress foregut development from blastula to early somite stages. (A) In situ hybridization of bisected Xenopus embryos (anterior left) shows that vent2 RNA is robustly expressed in the posterior endoderm from gastrula stage 11 to stage 22, in a reciprocal pattern to hhex. Throughout this period of development, wnt8 RNA is expressed in the lateral mesoderm (red arrows) adjacent to the vent2-expressing posterior endoderm. (B) Injection of vent2 RNA (500 pg) into the anterior D1 cells of the 32-cell stage embryo results in repression of foxa2 expression in the foregut endoderm at stage 18. (C) RNA encoding a hormone inducible GR-Vent2 fusion protein, with the hormone-binding domain of the glucocorticoid receptor fused to Vent2, was injected into the D1 anterior cells of 32-cell stage embryos. Dexamethasone (Dex, 10-6 M) was added to the media at the indicated stages, embryos were cultured until stage 35-37 and assayed by for1 and pdx1 in situ hybridization. Ectopic GR-Vent2 can repress foregut development up until stage 20, which is similar to the period when ectopically activated β-catenin can repress foregut development. No effect was observed on uninjected embryos treated with Dex, or on injected embryos without Dex.

Image published in: McLin VA et al. (2007)

Copyright © 2007. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

GeneSynonymsSpeciesStage(s)Tissue
foxa2.Lhnf3b, hnf3beta, hnf3-beta, HNF-3BX.laevisThroughout NF stage 18endoderm
foregut
neural tube
floor plate
hhex.LXHex, tHex, hexX.laevisSometime during NF stage 11 to NF stage 22endoderm
foregut
anterior
ventx2.2.Lvent-2, vent2, xvent2, xvent-2, XomX.laevisThroughout NF stage 11 to NF stage 22endoderm
posterior
wnt8a.LXwnt8, Xwnt-8, wnt-8, wnt8X.laevisSometime during NF stage 11 to NF stage 22mesoderm
lateral

Image source: Published

Larger Image
Printer Friendly View

Quality:
Good quality Poor quality
comment [+]
Return to previous page
Xenbase: The Xenopus laevis and X. tropicalis resource.
Version: 4.11.4


Major funding for Xenbase is provided by grant P41 HD064556