Xenbase Image ID: 49652

Fig. 3. The effect of Coco MO on asymmetrically expressed genes. Stage 256 embryos were stained by in situ hybridization for Pitx2c (A), Xnr1 (D), and Xlefty-a (G). Left and right sides of the same embryos are shown. Panels G are dorsal views of cleared embryos, showing midline expression of Xlefty-a. Embryos injected in the right dorsal blastomere with 3 ng Coco MO have mostly bilateral expression of Pitx2c (B), Xnr1 (E), and Xlefty-a (H). Coinjection of Mut Coco RNA (10 pg) restores left-side expression in Coco MO-injected embryos (Pitx2c C, Xnr1 F, Xlefty-a I). Panels A, D, G are controls. Embryos in panels A were coinjected with 1 ng LacZ RNA and stained with Red Gal, to show that the right side was correctly targeted. Image published in: Vonica A and Brivanlou AH (2007) Copyright © 2007. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue pitx2.L pitx2-A, pitx2-a, pitx2-b, pitx2c, xpitx2, xPtx2 X. laevis Sometime during NF stage 25 to NF stage 26 lateral plate mesoderm left nodal1.L nodal, nodal-related 1, nr1, nr1-a, spaw, Xnr-1, Xnr1 X. laevis Sometime during NF stage 25 to NF stage 26 mesoderm notochord lateral plate mesoderm left lefty.L antivin, atv, leftb, lefty-1, lefty-a, lefty-b, lefty1, leftyb, XATV, Xlefty X. laevis Sometime during NF stage 25 to NF stage 26 floor plate hypochord left Image source: Published Larger Image Printer Friendly View

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