Xenbase Image ID: 49788

Fig. 5. Xapelin and Xmsr knocked down embryos exhibited abnormal cardiovascular systems. (A, B) Western blotting assessing the specificities of XapelinMO and XmsrMO. XapelinMO (40 ng) and XmsrMO (20 ng) inhibited translation of mRNAs with 5′ UTR sequences (250 pg) (lane 2), but not from mRNAs that lack the 5′ UTR sequences (250 pg) (lane 4). (C, E, H) ControlMO (40 g)-, XapelinMO (40 ng)-, and XmsrMO (20 ng)-injected embryo at stage 45. (D, F, I) Section of the heart region of controlMO-, XapelinMO-, and XmsrMO-injected embryo at stage 45. Arrowheads indicate the position of the heart. (G, J) Embryos coinjected plasmid DNA (10 pg of pCS2-Xapelin 5m and 50 pg of pCS2-Xmsr 5m, respectively) with MOs (40 ng of XapelinMO and 20 ng of XmsrMO, respectively). (K, L, M) Graphs of the frequencies of edematous embryos in MO- and DNA-injected embryos. Three independent experiments were performed and showed similar results. The graphs show the representative results of one experiment. aMO: XapelinMO, mMO: XmsrMO, cMO: controlMO, Scale bar: 100 μm. Image published in: Inui M et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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