Xenbase Image ID: 49808

Fig. 10. Overexpression of Tbx20 in early Xenopus embryos. Tailbud embryos following radial (A) or ventral (B) injection with the following synthetic mRNAs; (A, B) HA-Tbx20a mRNA (500 pg) with LacZ mRNA (200 pg); (C) 200 pg LacZ mRNA; (D) HA-Tbx20b mRNA (500 pg) with LacZ mRNA (200 pg). (E) Western blot analysis of embryos injected with HA-Tbx20a or b using an anti-haemagglutinin (HA) epitope antibody. (F) Histological sections of HA-Tbx20a plus LacZ mRNA (left) or LacZ mRNA (right)-injected embryos at tailbud stage after staining for B-galactosidase. (G) Quantitative RT-PCR analysis of expression of indicated genes in total RNA isolated from whole Xenopus embryos at stage 13 or from animal caps removed at stage 9 from whole embryos that were uninjected or had been injected into the animal pole at the two-cell stage with HA-Tbx20a or b mRNA (250 pg) and cultured until sibling embryos reached stage 13. Expression levels are represented relative to those of the ubiquitously expressed ornithine decarboxylase (ODC) gene. Image published in: Stennard FA et al. (2003) Copyright © 2003. Image reproduced with permission of the Publisher, Elsevier B. V. Experiment + Assay Source Phenotypes and Disease Xla Wt + tbx20 + NF28 (whole-mount microscopy) Fig 10. A Anatomical Phenotype decreased length of anterior-posterior axis decreased length of tail Xla Wt + tbx20 + NF28 (whole-mount microscopy) Fig 10. A Anatomical Phenotype decreased length of trunk Xla Wt + tbx20 + NF28 (whole-mount microscopy) Fig 10. B Anatomical Phenotype abnormal eye abnormal whole organism abnormally abaxially mislocalized anterior foregut Xla Wt + tbx20 + animal cap explant + NF13 (RT-PCR) Fig 10. G Expression Phenotype increased amount a2m.L expression in animal cap increased amount nkx2-5.L expression in animal cap increased amount tbxt.L expression in animal cap decreased amount tubb2b.L expression in animal cap Xla Wt + tbx20 + NF28 (whole-mount microscopy) Fig 10. text Anatomical Phenotype abnormally abaxially mislocalized cardiac mesoderm abnormally duplicated cardiac mesoderm Xla Wt + tbx20 + animal cap explant + NF13 (RT-PCR) Fig 10. text Expression Phenotype increased amount ventx2.2.L expression in animal cap Larger Image Printer Friendly View

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