Xenbase Image ID: 49825

Fig. 4. Npnt knockdown phenocopies xdm. Npnt morpholino antisense oligonucleotides were injected into one blastomere of two-cell wild type embryos. (A, ventral view) Injection of two different non-overlapping morpholino sets resulted in unilateral forelimb ablation in a small but statistically significant number of metamorphosing tadpoles. (B) Morpholino knockdown ablating all forelimb skeletal elements, recapitulating the xdm phenotype. (C) Upper lanes, RT-PCR of control (c) and npnt splice-blocking (n) morpholino injected embryos indicates that npnt knockdown is virtually complete at stages 24 and 37, recovering at stage 42, and approaching wt levels at stage 45. Lower lanes, odc control. Image published in: Abu-Daya A et al. (2011) © 2011 Elsevier Inc. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Experiment + Assay Source Phenotypes and Disease Xtr.npntinsZimml + npnt MO + NF64 (stained tissue microscopy) Fig 4. A, B Anatomical Phenotype absent forelimb Xtr.npntinsZimml + npnt MO + NF64 (stained tissue microscopy) Fig 4. B Anatomical Phenotype abnormal pectoral girdle absent forelimb skeleton Larger Image Printer Friendly View

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