Xenbase Image ID: 72292

Figure S3. Effect of the modulation of Notch signaling on the development of NC and surrounding tissues. (A) Design of the Delta1 MO. The Delta1 translation start site is indicated in red (a). Delta1 MO inhibits the translation of both Delta1 pseudoalleles (b). In vitro transcription-translation reactions were performed by using the Sp6 Rabbit Reticulocyte Lysate System (Promega) in the presence of 35S methionine. Translation products were analysed by SDS-PAGE/autoradiography. (B) Whole-mount in situ analysis of embryos injected with Delta1 mRNA (1 ng), DeltaStu mRNA (1 ng) or Delta1 MO (7,5 ng) as indicated. The antisense probe used are indicated on the left. Note that Delta1 overexpression represses N-tubulin and increases Snail2 and Sox10. Conversely, N-tubulin is increased in Delta1Stu mRNA or Delta1 MO injected embryos (4 ng) and Snail2 and Sox10 is reduced. With the exception of Msx1 which is slightly reduced by the injection of DeltaStu mRNA or Delta1 MO, the other tested markers were unaffected by modulation of Delta1. Frequency of embryos with the indicated phenotype: a, 89% ectopic, n = 19; b, 57% reduced, n = 21; c, 60% reduced, n = 16; d, 45% increased, n = 20; e, 46% reduced n = 22; f, 67% reduced n = 18; g, 100% normal, n = 17; h, 82% normal n = 22; i, 72% normal, n = 22; j, 75% normal, n = 32; k, 77% normal, n = 22; l, 80% normal, n = 20 ; m, 100% normal, n = 26; n, 35% slightly reduced, n = 20; o, 40% slightly reduced, n = 24; p, 100% normal, n = 20; q, 100% normal, n = 18 ; r, 91% normal, n = 11; s, 100% normal, n = 10; t, 100% normal, n = 14 ; u, 100% normal, n = 16; v, 63% decreased, n = 19 ; w, 73% increased, n = 11; x, 38% increased, n = 8. Neurula stage embryos are viewed from the dorsal side, anterior down, with the injected side oriented to the right. Control and injected sides of tailbud embryos are shown. LacZ was used as a lineage tracer. Image published in: Nichane M et al. (2008) Copyright © 2008. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue sox10.L X. laevis Throughout NF stage 28 neural crest otic vesicle pharyngeal arch branchial arch migratory neural crest cell snai2.L slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. laevis Throughout NF stage 17 neural crest sox15.L sox-d, sox20, sox26, sox27, soxd, xsoxd X. laevis Throughout NF stage 17 neural plate pre-chordal neural plate chordal neural plate presumptive midbrain-hindbrain boundary pax3.S cdhs, hup2, pax-3, pax3-a, pax3-b, Waardenburg syndrome 1, ws1, xpax3 X. laevis Throughout NF stage 17 neural crest chordal neural plate border pre-chordal neural plate border msx1.L hox-7, hox7, msx-1, msx1-a, msx1-b, Xhox-7.1, Xmsx-1, xmsx1 X. laevis Throughout NF stage 17 neural crest pre-chordal neural plate border chordal neural plate border sox3.S Sry, Xlsox3, xSox3, Xtsox3 X. laevis Throughout NF stage 17 neural plate pre-chordal neural plate notoplate trigeminal placode six1.L XSix1 X. laevis Throughout NF stage 17 anterior neural ridge tubb2b.S N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 17 neuron Rohon-Beard neuron trigeminal placode Image source: Published Larger Image Printer Friendly View

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