Xenbase Image ID: 72293

Figure S3. Effect of the modulation of Notch signaling on the development of NC and surrounding tissues.(C) Q-PCR analysis of the expression of Snail2, Sox10, Sox3, Six1 and N-tubulin in NC induced caps analysed at the indicated stages and derived from embryos injected with Delta1 mRNA (500 pg/blastomere), DeltaStu mRNA (1 ng/blastomere) or Delta1 MO (5 ng/blastomere). Note that Delta1 overexpression upregulates Snail2 and Sox10 and that Delta1 inhibition diminished their expression. Note also that the expression of Sox3, Ep keratin, Six1 and N-tubulin was not significantly affected. (D) Phosphohistone H3 immunohistochemistry of neurula embryos injected with Delta1 mRNA (500 pg), Delta1Stu mRNA (1 ng) or Delta1 MO (7,5 ng) (top panels). Note the increase of pH3 staining in Delta1 injected embryos and the reduction of mitotic cells in Delta1Stu mRNA or Delta1 MO injected embryos (a, 38% increased, n = 21; b, 47% decreased, n = 19; c, 43% decreased, n = 21). Embryos are shown in dorsal view, anterior down, and the injected side is oriented to the right. Rhodamine dextran (red fluorescence) was used as a lineage tracer (bottom panels). Image published in: Nichane M et al. (2008) Copyright © 2008. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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