XB-IMG-72294
Xenbase Image ID: 72294
|
Figure S7. Hairy2-GR is unable to induce Delta1 expression in Stat3 depleted NC induced explants. (A) Stat3 MO (Ohkawara et al., 2004) inhibits the translation of Stat3. In vitro transcription-translation reactions were performed by using the Sp6 Rabbit Reticulocyte Lysate System (Promega) in the presence of 35S methionine. Translation products were analysed by SDS-PAGE/autoradiography.
(B) Injection of Stat3 MO (15 ng/blastomere) but not of a control Stat3 MO (Stat3 MOmis, 15ng/blastomere) blocks Hairy2 ability to induce Delta1 in NC induced explants as analysed by Q- PCR. Similar amount of the myc-tagged Hairy2-GR protein was produced in each condition as determined by western blot analysis. Image published in: Nichane M et al. (2008) Copyright © 2008. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |