XB-IMG-73827
Xenbase Image ID: 73827
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Fig.6. Induction of Nkx2.5 by FGF is mediated by p38 MAPK and not by ERK MAPK. (A) DMZ explants (n = 36) were dissected and at stage 11 were cultured in SU 5402 (30 μM) and allowed to develop to stage 16. Half of the explants were used to extract RNA for RT-PCR analysis (left) and the rest were used to extract proteins for Western analysis (right). (B) DMZ explants were cultured and processed as above, except their incubation in U0126 (30 μM). (C) DMZ explants were cultured and processed as above, except their incubation in SB203580 (30 μM). (D) Transcripts encoding FGF8 were injected and VMZ explants (n = 36) were dissected and gown from stages 11 to 15 in the absence or presence of SB203580 (30 μM). Half of the explants were used to extract RNA for RT-PCR analysis (left) and the rest were used to extract proteins for Western analysis (right). (E) Different combinations of transcripts encoding FGF8 and CREB AMO were injected to embryos, and VMZ explants were dissected at stage 10+. RNA was extracted at stage 15 and RT-PCR analysis was performed. Image published in: Keren-Politansky A et al. (2009) Copyright © 2009. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |