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Fig. 2. Perturbation of Notch signaling has variable effects on Shh expression. Shh expression in neurula (st.16, A–H) and tadpole (st.35, I–L) embryos upon blockade (B, F, J) and activation (C, D, E–H, K, L) of Notch signaling. Shh is expressed in the floor plate and notochord (A, A', I, I'). Blockade of Notch signaling led to a slight reduction in the floor plate domain of Shh in 31% of embryos (B, B'), but many embryos showed normal expression (J', 69%). Activated Notch signaling led to increased expression throughout the midline (C, C'), though the floor plate domain was normal (F, K'), in 27% of embryos. In a significant proportion of embryos (69%, p < 0.05), activated Notch led to a decrease in Shh expression throughout the midline (H, L') or in the floor plate domain (G). In NICD tadpoles, Shh expression was decreased and/or patchy in the spinal cord (K, L). The variable response of Shh to Notch activation is not correlated with amount or distribution of NICD protein (E–H, αnti-myc staining for NICD-myc in brown). NICD in the lateral neural plate does not cause ectopic Shh expression (E), while NICD in the FP domain can result in Shh expression (F), or repression (G, H). (A'–D') Transverse sections of (A–D); faint notochord expression of Shh is not visible in thin sections. (E–H) αnti-myc staining (brown) was performed on transversely bisected embryos after ISH for Shh (purple). The notochord is outlined in red and the ventral boundary of the neural plate in blue. (I–L) Lateral view of tailbud stage embryos. (I'–L') Transverse vibratome sections through middle of spinal cord at tailbud stages. Image published in: Peyrot SM et al. (2011) Copyright © 2011. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue shh.L hedgehog, vhh-1, X-SHH, Xhh, xshh X. laevis Throughout NF stage 16 notochord floor plate Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + dnrbpj + NF16 (in situ hybridization) fig.2.b, b^1 Expression Phenotype decreased amount shh.L expression in floor plate Xla Wt + canotch1 + NF16 (in situ hybridization) fig.2.c, c^1 Expression Phenotype increased amount shh.L expression in notochord Xla Wt + canotch1 + NF16 (in situ hybridization) fig.2.d, d^1 Expression Phenotype decreased amount shh.L expression in floor plate decreased amount shh.L expression in notochord Xla Wt + canotch1 + NF16 (in situ hybridization) fig.2.f Expression Phenotype increased amount shh.L expression in floor plate Xla Wt + canotch1 + NF16 (in situ hybridization) fig.2.g, h Expression Phenotype decreased amount shh.L expression in floor plate Xla Wt + canotch1 + NF16 (in situ hybridization) fig.2.k, l Expression Phenotype decreased amount shh.L expression in floor plate Larger Image Printer Friendly View

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