Xenbase Image ID: 73887

Fig.3. Shh is not a major regulator of dorsal midline fates. Shh signaling was blocked by injection of Shh MO or by cyclopamine treatment (“−Shh” B, E, G, J, M, P) and activated by injection of Shh mRNA (“+Shh,” C, H, K, N, Q). Netrin expression in the floor plate was decreased in 42% of − Shh embryos (B, N = 387) and slightly upregulated in 44% of + Shh embryos (C, N = 139). Floor plate expression of Shh was slightly narrower in 29% of embryos with blockade of Shh signaling (E inset, N = 194). Perturbation of Shh signaling had no effect on notochord (AxPC, F–H and Tor70, I–Q) or hypochord (O–Q) or floor plate markers F-spondin and FoxA2 in the spinal cord of tadpoles (I–N). (A–H) Dorsal views, anterior up, with transverse bisection in inset (A–E). (G–O) Transverse vibratome sections through middle of spinal cord at tailbud stages. Image published in: Peyrot SM et al. (2011) Copyright © 2011. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue shh.L hedgehog, vhh-1, X-SHH, Xhh, xshh X. laevis Throughout NF stage 16 notochord neural plate floor plate neural groove ntn1.L netrin 1, Netrin-1, ntn1l, unc-6 X. laevis Throughout NF stage 17 neural plate floor plate prechordal plate anterior neural fold pcdh1.L axial protocadherin, axpc X. laevis Throughout NF stage 14 notochord neural plate prechordal plate spon1.S F-spondin X. laevis Throughout NF stage 35 and 36 floor plate foxa2.L HNF-3B, hnf3-beta, hnf3b, hnf3beta, LOC108717763 X. laevis Throughout NF stage 35 and 36 floor plate vegfa.L vegf, vegf-a, vegfa-a, vegfa-b X. laevis Throughout NF stage 28 hypochord Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + Cyclopamine + NF17 (in situ hybridization) fig.3.b Expression Phenotype decreased amount ntn1.L expression in floor plate Xla Wt + shh MO + NF17 (in situ hybridization) fig.3.b Expression Phenotype decreased amount ntn1.L expression in floor plate Xla Wt + shh + NF17 (in situ hybridization) fig.3.c Expression Phenotype increased amount ntn1.L expression in floor plate Xla Wt + Cyclopamine + NF16 (in situ hybridization) fig.3.e Expression Phenotype decreased amount shh.L expression in floor plate Xla Wt + shh MO + NF16 (in situ hybridization) fig.3.e Expression Phenotype decreased amount shh.L expression in floor plate Larger Image Printer Friendly View

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