Xenbase Image ID: 73890

Fig.6. Shh and Notch signaling act in parallel to specify floor plate. Epistasis (A–L) and enhancement (M–P) experiments to determine the contribution of Notch and Shh signaling to floor plate formation. Embryos were injected and/or treated with reagents listed, fixed at stage 20–22, bisected and stained for co-injected lineage tracer (pink), then processed for Netrin ISH. Activated Notch signaling upregulates Netrin (B, F), while blockade of Shh signaling slightly reduces Netrin (C, G). Activation of Notch and blockade of Shh in the same embryo results in a Notch phenotype (D, H). Shh overexpression leads to a slight increase in FP (I), and blockade of Notch signaling leads to a decrease (J). Activation of Shh and blockade of Notch in the same embryo results in a Notch phenotype (K). Blockade of Notch signaling downstream of NICD with SDBM is able to rescue FP and notochord development to normal (L). Blockade (M, O) or activation (P) of both Notch and Shh signaling in the same embryo gives more severe phenotypes than perturbing either pathway alone. Image published in: Peyrot SM et al. (2011) Copyright © 2011. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue ntn1.L netrin 1, Netrin-1, ntn1l, unc-6 X. laevis Sometime during NF stage 20 to NF stage 22 floor plate neural tube Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + canotch1 + NF20-22 (in situ hybridization) fig.6.b, f Expression Phenotype increased amount ntn1.L expression in neural tube Xla Wt + shh MO + NF20-22 (in situ hybridization) fig.6.c Expression Phenotype decreased amount ntn1.L expression in floor plate Xla Wt + Cyclopamine + NF20-22 (in situ hybridization) fig.6.g Expression Phenotype decreased amount ntn1.L expression in floor plate Xla Wt + shh + NF20-22 (in situ hybridization) fig.6.i Expression Phenotype increased amount ntn1.L expression in neural tube Xla Wt + dnrbpj + NF20-22 (in situ hybridization) fig.6.j Expression Phenotype decreased amount ntn1.L expression in floor plate Larger Image Printer Friendly View

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