Xenbase Image ID: 73899

Fig.7. Siamois and Twin are required for Xwnt8 induction of ectopic axis formation. At the 4-cell stage both ventral blastomeres were injected with (C,D) a non-specific control morpholino oligonucleotide (NSMO, 50 ng), (E,F) a Sia-specific oligonucleotide (SiaMO, 25 ng), (G,H) a Twn-specific oligonucleotide (TwnMO, 25 ng), or (I,J) a combination of the Sia and Twn oligonucleotides (SiaMO + TwnMO, 25 ng + 25 ng). (B,D,F,H,J) At the 8-cell stage a single ventral blastomere was injected with Xwnt8 mRNA (5 pg). (A–J) Whole embryo morphology (dorsal up and, anterior right) is shown at the tailbud stage, with percentage of embryos displaying the representative phenotype and total embryos analyzed indicated in the lower right for each panel. (A) Uninjected control embryo. Image published in: Bae S et al. (2011) Copyright © 2011. Image reproduced with permission of the Publisher, Elsevier B. V. Experiment + Assay Source Phenotypes and Disease Xla Wt + wnt8a + NF32 (morphology) fig.7.b Anatomical Phenotype obsolete duplicated anterior-posterior axis Xla Wt + wnt8a + sia1 MO + NF32 (morphology) fig.7.f Anatomical Phenotype increased size of the ventral trunk obsolete duplicated anterior-posterior axis Xla Wt + wnt8a + sia2 MO + NF32 (morphology) fig.7.h Anatomical Phenotype obsolete duplicated dorsal-ventral axis Larger Image Printer Friendly View

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