Xenbase Image ID: 73901

Supplementary Figure. 3. Rescue of axial development in the Siamois-Twin double knockdown embryo. (B, D, F) At the 4-cell stage both dorsal blastomeres were injected with a combination of the Sia and Twn oligonucleotides (SiaMO + TwnMO, 25 ng + 25 ng). At the 8-cell stage a single dorsal blastomere was injected with 50 pg of (C, D) myc-Sia or (E, F) myc-Twn. myc-Sia and myc-Twn fully rescued axial development in double knockdown embryos (D, F), and resulted in mild dorsalization in control embryos (C, E). Whole embryo morphology (dorsal up and anterior right) is shown at the tailbud stage, with percentage of embryos displaying the representative phenotype and total embryos analyzed indicated in the lower right for each panel. (A) Uninjected control embryo. Image published in: Bae S et al. (2011) Copyright © 2011. Image reproduced with permission of the Publisher, Elsevier B. V. Experiment + Assay Source Phenotypes and Disease Xla Wt + sia1 MO + sia2 MO + NF31 (morphology) fig.S3.b Anatomical Phenotype absent head decreased length of anterior-posterior axis decreased size of the tail decreased size of the trunk Xla Wt + myc-sia1 + NF31 (morphology) fig.S3.c Anatomical Phenotype abnormal tail morphology decreased length of anterior-posterior axis decreased size of the tail Xla Wt + myc-sia2 + NF31 (morphology) fig.S3.e Anatomical Phenotype decreased length of anterior-posterior axis decreased size of the tail Larger Image Printer Friendly View

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