Xenbase Image ID: 73917

Fig.3. Comparative analysis of XtRIC-8 and control markers by whole mount in situ hybridization at early developmental stages of Xenopus tropicalis embryos. (A) The tissue specific expression pattern of ric-8 at different early stages of X. laevis and X. tropicalis embryonic development was analyzed by whole mount in situ hybridization. Probes are indicated in each panel at the left, stages are indicated at the top of each panel. XtRIC-8 antisense probe is shown on panels f–j, whereas XlRIC-8 probe is shown on panels from k to o. XtRIC-8 sense probe was used as negative control (panels a–e). Animal views are shown for embryos at stage 3, 6 and 8; vegetal views for embryos at stage 10 and dorsal views for embryos at stage 15 and 19. Animal–Vegetal (A–V) lateral view is shown in the inset at stages 3 and 6 (panels f and g). (B) The tissue specific expression of different control markers was determined at different developmental stages of X. tropicalis embryos by whole mount in situ hybridization. Sox-2, N-tubulin, EphA4, Twist, Slug and Xbra were used as control markers. Probes are indicated at the left side of each row and stages at the bottom-left side of each panel. Vegetal views are shown for stage 10; dorsal views for stage 15 and 19. Red arrows indicate specific structures that were labeled as: r (rhombomeres), nt (neural tube), nc (neural crest), mz (marginal zone), bl (blastopore lip), ppe (pre-placodal ectoderm) and np (neural plate). Image published in: Maldonado-Agurto R et al. (2011) Copyright © 2011. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue ric8a.S LOC108696733, ric-8, ric-8A, ric8a-a, ric8a-b, synembryn, xtric-8 X. laevis Throughout NF stage 3 (4-cell) animal hemisphere ric8a.S LOC108696733, ric-8, ric-8A, ric8a-a, ric8a-b, synembryn, xtric-8 X. laevis Throughout NF stage 6 (32-cell) animal hemisphere ric8a.S LOC108696733, ric-8, ric-8A, ric8a-a, ric8a-b, synembryn, xtric-8 X. laevis Throughout NF stage 10 marginal zone blastopore lip ric8a.S LOC108696733, ric-8, ric-8A, ric8a-a, ric8a-b, synembryn, xtric-8 X. laevis Throughout NF stage 15 brain neural crest neural tube neuroectoderm neural plate preplacodal ectoderm ric8a.S LOC108696733, ric-8, ric-8A, ric8a-a, ric8a-b, synembryn, xtric-8 X. laevis Throughout NF stage 19 brain neural crest neural tube neuroectoderm neural plate optic vesicle ric8a LOC108696733, ric-8, ric-8A, ric8a-a, ric8a-b, synembryn, xtric-8 X. tropicalis Throughout NF stage 3 (4-cell) animal hemisphere ric8a LOC108696733, ric-8, ric-8A, ric8a-a, ric8a-b, synembryn, xtric-8 X. tropicalis Throughout NF stage 6 (32-cell) animal hemisphere ric8a LOC108696733, ric-8, ric-8A, ric8a-a, ric8a-b, synembryn, xtric-8 X. tropicalis Throughout NF stage 10 blastopore lip marginal zone (sensu neural) ric8a LOC108696733, ric-8, ric-8A, ric8a-a, ric8a-b, synembryn, xtric-8 X. tropicalis Throughout NF stage 15 brain eye neural tube neuroectoderm neural plate preplacodal ectoderm eye primordium presumptive rhombomere ric8a LOC108696733, ric-8, ric-8A, ric8a-a, ric8a-b, synembryn, xtric-8 X. tropicalis Throughout NF stage 19 neural crest neural tube neural plate tubb2b N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. tropicalis Throughout NF stage 10 marginal zone tubb2b N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. tropicalis Throughout NF stage 15 neural crest neural tube neural plate sox2.L anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Sometime during NF stage 15 to NF stage 19 neuroectoderm neural plate anterior neural fold epha4 eph-a4, epha4-a, epha4-b, hek8, Pag, Pagliaccio, rtk1, sek, sek-1, tyr01, XEpha4 X. tropicalis Throughout NF stage 10 marginal zone (sensu neural) epha4 eph-a4, epha4-a, epha4-b, hek8, Pag, Pagliaccio, rtk1, sek, sek-1, tyr01, XEpha4 X. tropicalis Throughout NF stage 15 brain neural tube neuroectoderm eye primordium preplacodal ectoderm presumptive rhombomere rhombomere R3 rhombomere R5 tbxt bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. tropicalis Throughout NF stage 10 marginal zone marginal zone (sensu neural) tbxt bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. tropicalis Throughout NF stage 15 neuroectoderm neural groove neural tube anterior neural fold snai2 slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. tropicalis Throughout NF stage 15 neural crest neuroectoderm twist1 twist, twist1-a, twist1-b, X-twi, Xtwi, Xtwist X. tropicalis Throughout NF stage 15 neuroectoderm neural plate neural crest Image source: Published Larger Image Printer Friendly View

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