Xenbase Image ID: 73919

Fig.5. Comparative cross-section in situ hybridization analysis of XtRIC-8, N-tubulin and EphA4 in two developmental stages of X. tropicalis embryos. The tissue specific expression of XtRIC-8, the neuronal marker N-tubulin and the neural crest marker EphA4, were determined at stage 35 and 42 of X. tropicalis embryos by whole mount in situ hybridization. Embryos were coronally sectioned every 16–20 μm. The black lines in the upper panels indicate the position of the visualized sections. The probes are indicated at the top of the figure and the embryo stage at the bottom-left of each panel. Lateral views are shown for stage 35 embryos. The bottom panel shows a representation of the cross section of a stage 42 embryo. Red arrows indicate specific structures that were labeled as: nt (neural tube), l (lens), oc (otic capsule), ov (otic vesicle), and sm (somites). Image published in: Maldonado-Agurto R et al. (2011) Copyright © 2011. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue ric8a LOC108696733, ric-8, ric-8A, ric8a-a, ric8a-b, synembryn, xtric-8 X. tropicalis Throughout NF stage 35 and 36 eye neural tube brain hindbrain pharyngeal arch mandibular arch hyoid arch branchial arch otic vesicle forebrain ric8a LOC108696733, ric-8, ric-8A, ric8a-a, ric8a-b, synembryn, xtric-8 X. tropicalis Throughout NF stage 42 brain neural tube otic vesicle head somite hindbrain tubb2b N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. tropicalis Throughout NF stage 35 and 36 brain eye neural tube hindbrain spinal cord cranial ganglion tubb2b N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. tropicalis Throughout NF stage 42 brain eye neural tube hindbrain otic vesicle epha4 eph-a4, epha4-a, epha4-b, hek8, Pag, Pagliaccio, rtk1, sek, sek-1, tyr01, XEpha4 X. tropicalis Throughout NF stage 35 and 36 brain midbrain pineal gland hindbrain pronephric kidney neural tube chordoneural hinge pharyngeal arch lateral plate mesoderm heart primordium alimentary system stomodeal-hypophyseal primordium olfactory region rhombomere R3 rhombomere R5 epha4 eph-a4, epha4-a, epha4-b, hek8, Pag, Pagliaccio, rtk1, sek, sek-1, tyr01, XEpha4 X. tropicalis Throughout NF stage 42 neural tube otic vesicle Image source: Published Larger Image Printer Friendly View

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