Xenbase Image ID: 73956

Fig. 4. Bambi and smad6/7 negative feedback canalize development. (A) Phenotypes of stage-36/7 X. laevis embryos injected with the indicated MOs. (B) (Upper) Representative body and tail length of stage-36/7 X. laevis embryos injected with MOs as in A. (Lower) Average coefficient of variation of body and tail length of stage 36/7 X. laevis embryos injected as indicated in A of (n = 3) biological replicates with 15–30 embryos per replicate. (C) Eye morphology of stage-36/7 tadpoles from embryos injected as indicated. (D) Quantification of the relative eye size. Graph shows average eye area (Left) and coefficient of variation (Right) of three biological replicates with 15–30 embryos per replicate. (E) qPCR expression analysis of the indicated genes from individual X. laevis embryos of stage 10.5, which were injected at the four-cell stage with the indicated MOs (n = 9–10 embryos per sample). (F) Coefficient of variation of early BMP4 marker gene expression from individual stage 10.5 X. laevis embryos as in C (n = 2). Error bars, SD; *P < 0.05 and **P < 0.01 (unpaired t test). Image published in: Paulsen M et al. (2011) Copyright © 2011. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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