Xenbase Image ID: 74086

Fig. 2. Ectopic expression of Tbx2 inhibits pronephric nephron formation. (A-J) Xenopus embryos injected with Tbx2 (200 pg), Tbx2-GR (200 pg), VP16-Tbx2δC (100 pg) or EnR-Tbx2δC (100 pg) mRNA were treated (B,D,F) or otherwise (A,C,E,G-J) with dexamethasone (DEX) from stage 22 to 31 or 34 and then subjected to in situ hybridization for the pronephric markers Pax2, WT1 and Gremlin. The left and right images in each panel indicate the injected and uninjected control sides of the embryo, respectively. Of note, VP16-Tbx2δC disorganizes the pronephric tubules (G) and expands the glomus domain (I). (K) Tbx2 constructs used in the injection experiments. T-BOX, T-box from Tbx2; R, repressor domain of Tbx2; GR, human glucocorticoid receptor ligand-binding domain; VP16, transactivation domain of VP16; EnR, transrepression domain of Engrailed. Image published in: Cho GS et al. (2011) Copyright © 2011. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue pax2.L LOC108697493, pax-2, pax2-a, pax2-b, XPax-2, XPax2 X. laevis Sometime during NF stage 31 to NF stage 33 and 34 spinal cord pronephric duct pronephric tubule pronephric nephrostome wt1.S wt-1, wt1-a, wt1-b, Xwt-1, xwt1 X. laevis Throughout NF stage 33 and 34 pronephric kidney glomus grem1.L Cktsf1b1, cysteine knot superfamily 1, gremlin X. laevis Throughout NF stage 33 and 34 pronephric duct pronephric kidney intersomitic region Image source: Published Larger Image Printer Friendly View

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