Xenbase Image ID: 74087

Fig. 3. Loss of Tbx2 function expands the territory of the pronephric duct and glomus but not the tubule. (A-L) Xenopus embryos injected with Tbx2 MO (10 ng), Tbx2δC-GR (200 pg) or control MO (Co MO, 10 ng) were subject to in situ hybridization for the glomus-specific markers WT1 and Nephrin and the duct-specific marker Gremlin at stage 35 or the tubule-specific marker SMP30 at stage 31. To activate injected Tbx2δC-GR mRNA, embryos were treated with DEX from stage 22 to 35 (B,E) or to 31 (H). (A-I) Stained embryos are shown in the upper part of each panel and transverse sections at the levels indicated by the dashed lines are shown below. Left and right parts of each panel show the injected and uninjected control sides, respectively. cl, coelom; g, glomus; pd, pronephric duct; pt, pronephric tubule. Arrows in A,B indicate WT1 and Nephrin expression in the somatic layer of the intermediate mesoderm, respectively. Arrows and bracket in D,E indicate migrating Gremlin-expressing cells and the diameter of pronephric duct, respectively. (J-L) Control MO has no effect on the expression of Nephrin, Gremlin or SMP30. Image published in: Cho GS et al. (2011) Copyright © 2011. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue rgn.L regucalcin, smp30, xsmp-30, xsmp30 X. laevis Throughout NF stage 31 pronephric tubule wt1.S wt-1, wt1-a, wt1-b, Xwt-1, xwt1 X. laevis Throughout NF stage 35 and 36 glomus nphs1.S nephrin X. laevis Sometime during NF stage 31 to NF stage 35 and 36 glomus grem1.L Cktsf1b1, cysteine knot superfamily 1, gremlin X. laevis Sometime during NF stage 31 to NF stage 35 and 36 pronephric duct Image source: Published Larger Image Printer Friendly View

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