XB-IMG-74091
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Fig. 7. Gremlin and Hey1 downregulate Tbx2 by antagonizing BMP signaling. (A) Animal cap tissues from Xenopus embryos injected with Hey1 (100 pg) or Gremlin (10 pg) mRNA were subjected to RT-PCR analysis. (B-E) Embryos injected with Hey1 (100 pg) or Gremlin (10 pg) mRNA were subject to in situ hybridization for Tbx2, Gremlin or Hey1. Control shows the uninjected side of the embryo. Arrowheads (E) indicate Hey1 expression in the pronephros. (F) Animal caps from embryos injected with a combination of Bmp4 (200 pg), Gremlin (200 pg) and Tbx2 MO (10 ng) were treated with RA and activin for in vitro kidney induction and then subjected to RT-PCR analysis. W, whole embryo; AC, uninjected animal caps without RA and activin treatment; (–), uninjected animal caps with RA and activin treatment; –RT, negative control without reverse transcriptase; ODC, ornithine decarboxylase loading control. Image published in: Cho GS et al. (2011) Copyright © 2011. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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