XB-IMG-74109
Xenbase Image ID: 74109
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Fig. 2. Loss of PPan function strongly affects eye and craniofacial cartilage formation. (A) Schematic of Xenopus PPan protein illustrating the Brix domain (yellow) and σ70-like motif (red). Beneath is illustrated the σ70 deletion construct PPanδσ70. (B) Endogenous PPan is strongly reduced in cells derived from PPan MO-injected Xenopus animal caps (ACs), as compared with Control MO-injected ACs. Scale bars: 20 μm. (C,D) PPan MO1 and MO2, but not Control MO, injection results in a severe eye (C) and cranial cartilage (D) phenotype on the injected side (arrowheads and arrows) in a dose-dependent manner. Co-injection of δ5′UTR PPan RNA rescues PPan MO1-induced phenotypes, whereas PPanδσ70 does not. ba, branchial arches; mc, Meckel's cartilage; ta, tectum anterius. Quantitative evaluations are given to the right. n, number of independent experiments; N, total number of embryos examined. Error bars indicate s.e.m. Image published in: Bugner V et al. (2011) Copyright © 2011. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |