Xenbase Image ID: 74114

Fig. 7. PPan acts downstream of Wnt4. (A) PPan MO injection has no effect on Wnt4 expression at stage 13. (B) Representative Xenopus embryos displaying reduced expression of PPan at stage 13 upon bilateral injection of 20 ng Wnt4 MO (animal-dorsal, 8-cell stage). For qRT-PCR analyses, embryos were injected with noggin RNA (200 pg) and Wnt4 MO (3 ng) or c-Myc MO (10 ng) bilaterally at the 2-cell stage. Noggin-induced neuralized ACs were dissected and cultured to the equivalent of stage 13. Data are presented as relative units normalized to Control MO-injected samples. (C) Hypothesis I. PPan acts downstream of Wnt4 and upstream of Rx1, Pax6 and c-Myc at stage 13 in the context of eye and NC development. (D) Hypothesis II. PPan acts between Wnt4 and NC-specific genes during NC induction. (E) Unilateral PPan MO as well as Wnt4 MO injection interfere with Rx1, Pax6 and c-Myc expression on the injected side (black arrow, eye; red arrow, NC) at stage 13. By contrast, c-Myc MO has only a slight effect on Rx1 and Pax6 expression. (F) Wnt4, PPan and c-Myc depletion lead to a similar downregulation of the NC marker genes Slug, FoxD3 and Twist at stage 16/17. (G,H) Quantification of the experiments in E (G) and F (H). Co-injection of PPan RNA rescues Wnt4 MO-induced reduction of all analyzed eye and NC marker genes. By contrast, co-injection of c-Myc RNA does not revert PPan MO-induced effects. n, number of independent experiments; N, total number of embryos examined. Error bars indicate s.e.m. Image published in: Bugner V et al. (2011) Copyright © 2011. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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