XB-IMG-74115
Xenbase Image ID: 74115
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Fig. 8. PPan has a function in apoptosis. (A) Eight-cell stage Xenopus embryos were injected with PPan MO (5 ng) or Control MO (5 ng) into one animal-dorsal blastomere. TUNEL staining was used to label apoptotic cells. PPan depletion results in increased apoptosis on the manipulated side at stage 23 (arrow), which could not be rescued by co-injection of p53 MO (5 ng). Control MO-injected embryos were not affected. A quantitative evaluation is given beneath. (B) The increase in apoptosis can already be observed at stage 13. (C) Bilateral injection of 40 ng PPan MO at the 2-cell stage leads to increased activity of caspases 3 and 7. This effect can be reverted by co-injection of 4 ng human (h) BCL2 RNA. The same effect was observed upon injection (bilateral, animal-dorsal) of 10 ng PPan MO and 2 ng human BCL2 RNA at the 8-cell stage. Values represent relative light units (RLU) normalized to one Control MO-injected sample per series of experiments. (D) Co-injection of 1 ng human BCL2 RNA into one animal-dorsal blastomere at the 8-cell stage rescued the PPan MO-induced reduction of Rx1 expression (arrow). **, P<0.01; *, P<0.05. n, number of independent experiments; N, total number of embryos examined. Error bars indicate s.e.m. Image published in: Bugner V et al. (2011) Copyright © 2011. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |