Xenbase Image ID: 74116

Fig. 9. Cellular localization of PPan. (A) Immunofluorescence staining of human PPAN (hPPan) and UBF1 in HEK293 cells by use of specific antibodies. The overlay shows the colocalization (red arrows) of PPAN (green) and UBF1 (red). White arrows indicate extranuclear PPAN. Nuclei are visualized by DAPI staining (blue). (B) PPAN is located in the mitochondria of HEK293 cells as shown by counterstaining with MitoTracker Red CMXRos. (C) Neuralized AC cells equivalent stage 13 were dissociated and stained for PPan (green). Xenopus (X) PPan is localized to the nucleoli (red arrows) and in the cytoplasm (white arrows) of neural cells. (D) Immunofluorescence staining in attached cells from untreated ACs revealed that Xenopus PPan (green) can be found in the cytoplasm but not at the cell membrane, which is labeled by β-catenin staining (red). Scale bars: 10 μm in A,B; 20 μm in C,D. Image published in: Bugner V et al. (2011) Copyright © 2011. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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