Xenbase Image ID: 74151

Fig. 1. snail1 MO effects. Xenopus embryos were injected with RNAs encoding myc-tagged GFP (mt-GFP; 50 pg/embryo) and UTR-Snail1-GFP (which latter includes the target of the snail1 MO) RNAs (600 pg/embryo), either alone or together with the snail1 MO (7 ng/embryo). (A) Immunoblot analysis of stage 11 embryos using an anti-GFP antibody revealed a clear and specific reduction in the accumulation of Snail1-GFP as compared with GFP in the snail1 MO-injected sample. (B,C) UTR-Snail1-GFP RNA was injected into one cell of 2-cell embryos either alone (B) or together with the snail1 MO (C). At stage 11, the snail1 MO greatly reduced UTR-Snail1-GFP fluorescence. (D,E) RT-PCR (D) and qPCR (E) analyses indicate that the injection of the snail1 MO into both cells of 2-cell embryos led to a specific reduction in the levels of twist1 and snail2 RNAs at stage 11. Ornithine decarboxylase (ODC) RNA was used to control for non-specific effects. Error bars indicate s.d. Image published in: Shi J et al. (2011) Copyright © 2011. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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