Fig. 2. snail1 MO effects on germ layer markers. (A-B′) snail1 MO injection (into one cell of a 2-cell embryo) led to the loss of expression (as measured by in situ hybridization) of xbra (A′, versus control in A) and to an increase in endodermin (edd) (B′, versus control in B) RNA levels (at stage 11). (C,C′) Section analysis revealed that the edd expression domain, which is restricted to the superficial region in control embryos (C), extends deeper into the mesodermal region in snail1 MO-injected embryos (C′, black arrow indicates lacZ marker staining, and the red arrow indicates the extent of edd staining in deep mesodermal tissue). (D-F′) There was a loss of expression of the neural crest/placodal marker sox9 at stage 17/18 (D, severely affected; E, mildly affected; injected sides to the right), as well as a loss of expression of myoD at stage 25, as expressed in myotomal muscle, a mesodermal derivative (F′, versus control in F). (G-J) The effects on sox9 (G,H) and myoD (I,J) of snail1 MO injection (G,I) were rescued by the injection of the Snail1-GFP RNA (H,J), which lacks the snail1 MO target sequence. Injected sides are shown. (K) The percentage of normal embryos plotted with respect to xbra, edd, myoD and sox9 in situ expression. Blue, snail1 MO injected; green, snail1 MO together with snail1 RNA (600 pg/embryo); yellow, snail1 MO together with Snail2-GFP RNA (600 pg/embryo); red, snail1 MO together with Twist1-GFP RNA (600 pg/embryo). The number of embryos examined is indicated above each bar.
When using the Xenbase gene expression search we felt it would be most valuable if high quality images appeared near the top of your search results. That is why we have developed a way to allow Xenbase users to vote on the quality of an image. You can change your vote for a given image as many times as you want, but only your last vote is counted. Additionally,we've provided a comment box if you want to tell us why you think a specific image is good or bad.
As you can see above, this information box will tell you how people have voted on the image you are currently looking at. It is important to note that all of your votes are completely anonymous.
[2.8.5]
