Xenbase Image ID: 74182

Figure 2. Dox-induced GFP expression varied across and within founder offspring. Five-day-old tadpoles transgenic for pDPCrtTA-TREG-HS4 were treated with 50 μg/mL Dox for 3 days and shown under brightfield and green fluorescence microscopy. A: All transgenic offspring from each founder lacked visible GFP expression in the absence of Dox, and showed induction of GFP in the presence of Dox. An exemplar tadpole from ♂8 is shown. B: The offspring from four founders (♀1, ♂8, ♂6, ♂1) vary in GFP brightness, likely due to chromosomal insertion site effects. C: A range of Dox-induced GFP expression levels was observed among transgenic sibling offspring from some founders. F1 offspring from ♂8 are shown. A non-transgenic tadpole (left) is shown for comparison.Download figure to PowerPoint Image published in: Rankin SA et al. (2011) Copyright © 2011. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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