XB-IMG-74201
Xenbase Image ID: 74201
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Fig. 6. BMP depletion rescues tsg morphants morphologically. (A) The incidence of blastopore closure was quantified in embryos injected at the two- cell stage with tsg MO, BMP MOs, or varying combinations of tsg and BMP MOs as indicated. The experiment was repeated with similar results. (B) Co- injection of tsg MO with BMP MOs rescues embryos morphology to varying extents. Embryos were injected at the 2-cell stage with 10 ng of tsg MO and either 40 ng of BMP4 MO, 40 ng of BMP7 MO, or 20 ng of both BMP4 and BMP7 MOs. For each combination, the percentage of embryos observed with each degree of rescue was quantified. Representative embryos for each category of rescue are included at right. The category represented in blue indicates embryos with minimal or no rescue of head morphology or embryo length. Embryos represented by the red category had mild to moderate rescue of head morphology and/or embryo length. Embryos represented by the yellow category showed significant rescue of head morphology, embryo length and overall morphology. For panels A and B, all embryos were injected at the 2-cell stage with the combination of MOs indicated, and were assayed at stage 25 for blastopore closure and morphology. Image published in: Wills A et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |