Xenbase Image ID: 74269

Fig.2. The HES genes esr9 and esr10 are Notch-responsive in marginal zone mesoderm during gastrulation. All images are gastrula stage whole embryos viewed from the vegetal pole. (A, D) In situ hybridization analysis of uninjected embryos shows that the expression of the putative downstream Notch effector genes, esr9 and esr10, are expressed in the marginal zone in a ring pattern coinciding with delta, and xotch expression (Fig. 1). Embryos were co-injected with a lineage label and either GR-Su(H)VP16 to upregulate Notch signaling (B, E) or GR-Su(H)DBM to downregulate the Notch pathway (C, F). Constructs were induced at the onset of gastrulation by the addition of dexamethasone to the culture medium and collected 1 h later. Arrowheads indicate the location of the progeny of the injected cells as detected by the presence of magenta-gal-positive nuclei. (B, E) An expansion of esr9 and esr10 expression resulted when Notch signaling was upregulated (esr9 78% of embryos, n =  120; esr10 82% of embryos, n =  82) and decreased expression was observed when Notch signaling was downregulated (esr9 68% of embryos, n =  96; esr10 75% of embryos, n =  52). On average, 21% or less of both uninjected-control embryos and injected embryos not exposed to hormone were observed to contain alterations in normal esr9 and esr10 expression (normal esr9 expression was observed in: 91% of embryos injected with GR-Su(H)VP16/no hormone, n =  104; 85% of embryos injected with GR-Su(H)DBM/no hormone, n =  128; normal esr10 expression was observed in: 91% of embryos injected with GR-Su(H)VP16/no hormone, n =  57; 79% of embryos injected with GR-Su(H)DBM/no hormone, n =  91). Percents represent the number of embryos with the phenotype described resulting from three replicate experiments conducted on different days. Image published in: Miazga CM and McLaughlin KA (2009) Copyright © 2009. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue hes5.6.L X. laevis Throughout NF stage 10.5   hes5.5.S esr10 X. laevis Throughout NF stage 10.5   Image source: Published Larger Image Printer Friendly View

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